var
 aBitmap: TIEBitmap;
begin
 aBitmap := TIEBitmap.Create;
  try
   aBitmap.Allocate( ImageEnView1.Width, ImageEnView1.Height );
   ImageEnView1.LayersDrawTo( aBitmap );
   aBitmap.Write( sFilename );
  finally
   FreeAndNil( aBitmap );
  end;
end;

procedure TFormChromaKey.FileListBoxGreenDoneChromeClick(Sender: TObject);
var
    BackGroundFileName, ForeGroundFileName : String;
    Tolerance: Double;
begin
    ForeGroundFileName := FileListBoxGreenDoneChrome.Directory + '\' + FileListBoxGreenDoneChrome.Items[FileListBoxGreenDoneChrome.ItemIndex];
    ImageEnViewForeground.IO.LoadFromFileJpeg(ForeGroundFileName);
    If DM1.dbTableReceivedEmail.FindKey([JustFileNameS(ForeGroundFileName)]) then begin
       BackGroundFileName := cfGSImageFolder + '\'
                            + DM1.dbTableReceivedEmail.FieldByName('PackageCode').AsString + '\'
                            + JustFileNameS(DM1.dbTableReceivedEmail.FieldByName('BackGroundFileName').AsString);
       If FileExists(BackGroundFileName) then begin
          ImageEnViewBackGround.IO.LoadFromFileJpeg(BackGroundFileName);
          ImageEnView1.LayersCurrent := 0;
          ImageEnView1.IO.LoadFromFile(BackGroundFileName);
          ImageEnView1.LayersCurrent := 1;
          fForegroundSrc.Read(ForeGroundFileName);
          if fForegroundSrc.Width > 1024 then fForegroundSrc.Resample( 1024, -1 );
          ImageEnView1.Deselect;
          ImageEnView1.Layers[ 1 ].Bitmap.Assign( fForegroundSrc );
          ImageEnView1.Deselect;
          ImageEnView1.Layers[ 1 ].Bitmap.Assign( fForegroundSrc );
          KeyColor := ImageEnView1.Proc.GuessChromaKeyColor( Tolerance );
          DelayedApplyChromaKey();


  // It works with below showmessage  // i tryed placing pauses in here but didn't work
          Showmessage('xyz');

  // If I remove showmessage it saves but the foreground loses it's transparency

          SaveTransParentLayers('C:\GoodEyeOw\ALL-GREEN\Done\' + JustFileNameS(ForeGroundFileName));
          end;
       end;
end;

// routine you sent
procedure TFormChromaKey.SaveTransParentLayers(SFileName: String);
var
   aBitmap: TIEBitmap;
begin
   aBitmap := TIEBitmap.Create;
   try
    aBitmap.Allocate( ImageEnView1.Width, ImageEnView1.Height );
    ImageEnView1.LayersDrawTo( aBitmap );
    aBitmap.Write(sFileName);
    finally
    FreeAndNil( aBitmap );
    end;
end;

/////////////////////////////////////////////////////
This button DOESN'T WORK

procedure TFormChromaKey.Button2Click(Sender: TObject);
begin
    FileListBoxGreenDoneChromeClick(Sender);  // will 
    SaveTransParentLayers('c:\testimage.jpg');
end;

///////////////////////////////////////////////////
This button WORKS if I run FileListBoxGreenDoneChromeClick(Sender);  first without showmessage;

procedure TFormChromaKey.Button2Click(Sender: TObject);
begin
    SaveTransParentLayers('c:\testimage.jpg');
end;